Info-enriched characterization of mobile populations. Morphological details from illustrations or photos can incorporate on the richness of flow cytometry details. One example is, the figure displays an if not regular apoptosis assay utilizing Annexin V and PI, incorporating mobile imaging to characterize cells in Each individual populace to reveal morphologically unique characteristics. These insights could not are obtained from multiplex staining by itself.

As samples are acquired about the Attune CytPix Flow Cytometer, the large-velocity brightfield digital camera captures and outlets visuals of detected occasions in a amount as much as six,000 photographs for every 2nd, depending upon the flow price and impression dimensions. For increased versatility, Attune Cytometric Application allows you to alter the impression capture frequency as desired and select a certain gate for impression seize, if required.

(B) RAW 264.7 macrophages are frequently annotated inaccurately with regard to particle depend because of their complexity and noncircular borders. The quantity of singlets accessible for Investigation will increase as soon as the person trains and employs a more exact model.

Find new insights into your experimental knowledge by visualizing populations making use of merged fluorescence and morphology data. Our application interface wizards allow downsampling, concatenation (merged documents) and dimensionality instruments that happen to be crafted into the application for an intuitive, streamlined knowledge Investigation encounter.

"An image is not very worth one thousand dots, but it truly really helps to see photos. It is excellent to see visuals that correlate with staining. By way of example, AnnexinV-favourable cells that happen to be morphologically diverse than healthful cells, or CD14+ monocytes that are greater and a lot more textured than CD3+/CD4+/CD14- lymphocytes.

Furthermore, it's illuminating to see the number of doublets slip by means of standard singlet gates, and wonderful to confirm the position of unpredicted double-positive situations in my Assessment."

Pair your Attune flow cytometer with the autosampler for enhanced workflow effectiveness, and include robotic integration for maximum throughput.

Steady image excellent even at large throughput Acoustic focusing along with a significant-pace camera combine to picture these T cells constantly at lower or high flow prices. Quickly regulate emphasis and camera options to fulfill experimental demands.

The Attune NxT cytometer may be the trusted workhorse instrument your lab can depend upon for constant and trusted functionality. The Attune CytPix model includes a superior-speed brightfield digicam and AI-pushed graphic Investigation software, with latest click here upgrades including device Studying instruments and an intuitive user interface, enabling Increased data precision, accuracy, and more rapidly insights into sample biology.

End users can watch function visuals in true-time to confirm sample excellent, enhance protocols, and document the morphology of each and every mobile populace discovered for Evaluation. Imaging seize also Rewards from acoustic focusing, which helps you to posture the cells to ensure that a sharp, centered image is attained.

AI-driven automatic picture analysis With all the Attune Cytometric Software interprets event functions into distinctive morphology parameters which might be coupled with regular fluorescence and scatter parameters to expose very reproducible populace improvements and unique subsets.

Application Observe: Automated graphic Assessment minimizes consumer-to-consumer variability in flow cytometry gating techniques

Leverage the power of State-of-the-art technological know-how and innovation to accelerate your discoveries. Have confidence in within the Attune Flow Cytometer relatives to deliver the reliability, velocity, and precision that set your do the job aside.

No wash/no lyse leukocyte Investigation with imaging. Cells have been acquired from 24-hour-old blood by dilution in one mM EDTA ( Specialist UX/UI enables end users to coach AI products for expanded apps. (A) The “Coach Product” element delivers annotation instruments and actual-time design education accuracy.

Gating on these extended parameters permits you to promptly and correctly recognize populations of desire and confirm gating strategy with little if any handbook evaluation. The Attune software features access to condition-of-the-artwork equipment Studying Examination tools inside of an expertly made person interface, making it extremely simple to operate.

Because the cell line had Earlier been analyzed within the Attune CytPix Flow Cytometer, the scientists went back to the images and were in a position to document the microbial an infection at the least five times before.

Agilent Benefit Guarantee ensures you no less than 10 years of instrument use in the date you purchase. Normally, We are going to credit history you Along with the residual value of the program towards an upgraded product.

What gave the impression to be a homogeneous populace is definitely a lot more varied—an Perception that needs to be regarded as when interpreting effects.